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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial role in advertising cell-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are described. Two supplemental splice variants ended up claimed but thorough nucleotide sequence info is not really nevertheless out there.
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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide generally has the epitope recognized through the antibody.
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This information outlines more feasible optimization of mobile and immunoassay parameters to guarantee the absolute best success are...
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This guidebook outlines even further probable optimization of mobile and immunoassay parameters to ensure the absolute best benefits are...
Antibodies certain to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, one can see which staining is unique.
Concentrate on Information and facts Cyclin E belongs to the highly conserved cyclin family, whose associates are characterised by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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